There are two questions being asked here.

1. Does Windows 2000 multiple IP addresses?

Yes. In the Protocol dialog where you assign one IP address, there should be an "Advanced" button which opens a dialog box for assigning multiple IP addresses to the one network card. I've seen more than 20 addresses on the one card in Windows 2000!

2. Can a second MySQL instance be run and listen on a different IP address?

Yes. [link|http://www.mysql.com/doc/C/o/Command-line_options.html|There is a command-line option to do this] (--bind-address) which you may need to put in the Service entry. It seems you might be able to put this setting into a my.cnf file, but the documentation doesn't say.

Wade.